BBa_K1444009

BBa_K1444009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1444009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and consensus B.subtilis RBS - C1-434 x3

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1444009_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the copies of the repressible promoter C1-434 (BBa_R0052), and ending with the consensus RBS from B. subtilis (BBa_K090505).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public	BBa_K823003	BBa_K823003
public	BBa_R0052	cI 434
public	BBa_R0052	cI 434
public	BBa_R0052	cI 434
public	BBa_K090505	BBa_K090505

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003	1,237	BBa_K823003
BBa_R0052	246,291	cI 434
BBa_R0052	300,345	cI 434
BBa_R0052	354,399	cI 434
BBa_K090505	408,418	BBa_K090505

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1444009/1

Member of these Collections

• iGEM Parts Registry