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BBa_K1444013

BBa_K1444013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1444013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and weak B. subtilis RBS - pTetR

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Details

Types	DnaRegion
Roles	Regulatory promoter
Sequences	BBa_K1444013_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the repressible promoter pTetR (BBa_R0040), and ending with the weak RBS from B. subtilis (BBa_K780002).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pTetR repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public public public	BBa_K823003 BBa_R0040 BBa_K090505	BBa_K823003 p(tetR) BBa_K090505

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003 BBa_R0040 BBa_K090505	1,237 246,299 308,318	BBa_K823003 p(tetR) BBa_K090505

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1444013/1