BBa_K1445002

BBa_K1445002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1445002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Josephina Hendrix

Date created: 2014-09-24 11:00:00

Date modified: 2015-05-08 01:10:27

High copy BioBricked M13 phagemid vector

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K1445002_sequence (Version 1)

Description

This biobrick backbone has the high copy number pUC19 origin of replication and an Ampicillin resistance marker.

Part also contains the M13 origin of replication which allows the phagemid to be taken up by the M13 phage. Vector does not contain any expressible phage genes; therefore, a Helper Phagemid is required for phage production.

Vector contains the following:
iGEM VF2 and VR sequencing sites
T7 promoters that flank the MCS
Translational stop sequences (BBa_B0042) that flank the MCS
Transcriptional terminator BBa_B0055 upstream of the MCS
Transcriptional terminator BBa_B0054 downstream of the MCS

Notes

Plasmid was designed to have the iGEM VF2 and VR sequencing sites along with translational and transcriptional terminators flanking the MCS.

Source

Limtus28i phagemid vector from NEB. Areas flanking MCS were cloned from pSB3C5 backbone.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1445002/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors