BBa_K1448008

BBa_K1448008 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1448008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: yuma kobayashi

Date created: 2014-10-15 11:00:00

Date modified: 2015-05-08 01:10:28

In E coli mono-terpene production cassette

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K1448008_sequence (Version 1)

Description

This part contains the IPTG inducible tac promoter, RBS and erg20-2 generator (BBa_K1448007).
Usage
How to use
First, digest this part with SpeI
Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.
Third, mix and ligate both samples.

Various monoterpenes can be produced in E. coli cells containing this device and monoterpen synthase genes without exogenous GPP.

Notes

How to use
First, digest this part with SpeI
Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.
Third, mix and ligate both samples.

Source

BBa_K1448007 from our design part.
IPTG inducible tac promoter from pGEX-6P-2

Components

Access	Instance	Definition
public	BBa_K1448007	BBa_K1448007

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1448007	1,2703	BBa_K1448007

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1448008/1

Member of these Collections

• iGEM Parts Registry