Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: yuma kobayashi
Date created: 2014-10-18 11:00:00
Date modified: 2015-05-08 01:10:28
In E coli mono-terpene production cassette
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1448013_sequence (Version 1) |
Description
This part contains the IPTG inducible tac promoter, RBS and erg20-2 generator (BBa_K1448007).First, digest this part with SpeI.
Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.
Third, mix and ligate both samples.
Various monoterpenes can be produced in E. coli cells containing this device and monoterpene synthase genes without exogenous GPP.
Notes
First, digest this part with SpeI.Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.
Third, mix and ligate both samples.
Various monoterpenes can be produced in E. coli cells containing this device and monoterpene synthase genes without exogenous GPP.
Source
This is a composite part consisting of K314110, K314111, R0011, B0034 and K517005.| Access | Instance | Definition |
| public public | BBa_K1448007 BBa_B0034 | BBa_K1448007 BBa_B0034 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1448007 BBa_B0034 | 1,2703 2712,2723 | BBa_K1448007 BBa_B0034 |