GFP

BBa_K145015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K145015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Benjamien Moeyaert

Date created: 2008-07-31 11:00:00

Date modified: 2015-05-08 01:10:28

GFP with LVA tag

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K145015_sequence (Version 1)

Description

Green Fluorescent Protein with LVA tag for rapid degradation

Notes

Designed out of pJBA111 (Molin, 1998) with PCR to add appropriote prefix and suffix.

Source

pJBA111, carrying GFP-LVA, was generously provided by Dr. S. Molin, The
Technical University of Denmark, Lyngby

Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
PMID: 9603842

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	feature/start, start_codon
stop	754,756	stop_codon, feature/stop
stop	757,759	stop_codon, feature/stop
LVA tag	717,753	tag, feature/tag
cds	4,716	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K145015/1

Member of these Collections

• //function/reporter/fluorescence
• iGEM Parts Registry