BBa_K145150

BBa_K145150 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K145150

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonas Demeulemeester

Date created: 2008-08-05 11:00:00

Date modified: 2015-05-08 01:10:29

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K145150_sequence (Version 1)

Description

Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 O_R2 and O_R1 are located between the -35 and -10 boxes and downstream of them respectively.

Notes

-35 box is partly composite. -10 is taken from the P22 phage P_R regulatory region

Source

Synthetic

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Lux-box	1,20	non_covalent_binding_site, feature/binding
-35	20,25	promoter, feature/promoter
OR2	23,40	non_covalent_binding_site, feature/binding
-10	42,47	promoter, feature/promoter
OR1	46,63	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K145150/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //function/cellsignalling/luxr
• //promoter
• //regulation/multiple
• //regulation/negative
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry