BBa_K1452000

BBa_K1452000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1452000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Keoni Gandall
Date created: 2014-11-02 12:00:00
Date modified: 2015-06-01 02:28:12

Pos/neg selection (Bacillus)



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1452000_sequence (Version 1)

    Description

    This is a positive/negative selection cassette for Bacillus. If flanked by Bacillus integration sites and integration sites for any chosen DNA, that DNA can be integrated into Bacillus and then the selection cassette can be integrated over by whatever DNA you chose.

    Notes

    ***the mazF toxin is unstable in plasmids without the mazE antitoxin***

    Source

    http://www.bgsc.org/viewdetail.php?bgscid=ECE194&Search=pDGieF
    http://www.ncbi.nlm.nih.gov/pubmed/16714443

    Access Instance Definition
    public
    		BBa_K302033
    		BBa_K302033

    Sequence Annotation Location Component / Role(s)
    BBa_K302033
    		1,339
    		BBa_K302033

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1452000/1
     

    Attachments

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