Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Renhe Luo
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:29
ssDsbA-mRFP-Lgt-TAL USB-His Tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1453000_sequence (Version 1) |
Description
This part is designed as a scaffold of connectee.The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000
The TAL adapter part contains T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI, in order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), which is designed by iGEM12_Freiburg.
Notes
When digest with BsmBI, this part can create two sticky-ends, one can match with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, the other can match with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), user can synthesize a 14 nucleotide Transactivator-like (TAL) protein to suit for their own connector and design their own polymerization. (Golden Gate Cloning)
More details about membrane anchor, please find http://2012.igem.org/Team:SJTU-BioX-Shanghai.
More details about TAL and Golden Gate Cloning, please find http://2012.igem.org/Team:Freiburg.
Source
BBa_K771000BBa_E1010
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1453000 ssDsbA mRFP HL Lgt FL TAL USB His TAG TAA ATG | 1,2163 1,57 58,732 733,780 781,1653 1654,1710 1711,2142 2143,2160 2161,2163 1,3 | engineered_region feature/BioBrick CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein feature/dna sequence_feature tag feature/tag stop_codon feature/stop feature/start start_codon |