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BBa_K1453006

BBa_K1453006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1453006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Renhe Luo

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:29

TAL USB-His Tag

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1453006_sequence (Version 1)

Description

This part is designed as a scaffold of the second kind of connectee, which can anchor on the membrane.

In order to bind TAL protein designed by 2012 Freiburg iGEM team, the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.

When digested with BsmBI, this part can produce two sticky-ends that can bind TAL-Protein DiRepeat (Bba_K747000 to Bba_K747095)

Notes

More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.

Source

T1 sequence, T14 sequence

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG BBa_K1453006 FL Tal USB His Tag TAA	1,3 1,513 4,60 61,492 493,510 511,513	feature/start start_codon feature/BioBrick engineered_region CDS feature/protein sequence_feature feature/dna feature/tag tag stop_codon feature/stop