BBa_K1453404

BBa_K1453404 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1453404

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yangyang Li, Binhan Hao, Renhe Luo

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:30

ssDsbA-Lgt-pykF-TAL1-His Tag

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1453404_sequence (Version 1)

Description

This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.
The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.

Notes

This part can recognize a special sequence, T-TCGATATCAAGC-T.
This part contains the pyruvate kinase (EC:2.7.1.40).

Source

E.coli, BBa_K1453403, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1453404	1,3951	engineered_region, feature/BioBrick
ATG	1,3	start_codon, feature/start
ssDsbA	1,57	feature/protein, CDS
HL	58,105	CDS, feature/protein
Lgt	106,978	feature/protein, CDS
pykF	1036,2445	feature/protein, CDS
BBa_K1453007	2446,3951	feature/BioBrick, engineered_region
FL	979,1035	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1453404/1

Member of these Collections

• iGEM Parts Registry