Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yangyang Li, Binhan Hao, Renhe Luo
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:30
pykF-TAL1-His Tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1453408_sequence (Version 1) |
Description
This part is used in our application test free in the cytoplasm. See more details please view iGEM14_SJTU_BioX_Shanghai.This part is based on the BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.
Notes
This part can recognize a special sequence, T-TCGATATCAAGC-T. This part contains the pyruvate kinase (EC:2.7.1.40).Source
E.coli, BBa_K1453406, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.| Sequence Annotation | Location | Component / Role(s) |
| pykF ATG BBa_K1453408 BBa_K1453007 | 1,1410 1,3 1,2916 1411,2916 | CDS feature/protein feature/start start_codon engineered_region feature/BioBrick engineered_region feature/BioBrick |