BBa_K1457000

BBa_K1457000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1457000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michelle Long, Nicholas Larus-Stone, Jonah Saltzman, Tiana Raphel

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:32

mRFP_Spycatcher

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1457000_sequence (Version 1)

Description

This part is the coding sequence for a modified red fluorescent protein fused to 'Spycatcher' protein. Spycatcher specifically amide bonds to an orthogonal polypeptide sequence called 'Spytag'. This part can be used to bind and detect any engineered constructs displaying Spytag. In our specific system, it is engineered to bind csgA protein fused to spytag and is applied extracellularly to easily detect CsgA production.

Notes

This part is a direct fusion of mRFP and the Spycatcher sequence. We would have liked to include a linker between the two sequences in the original design.

Source

mRFP from team Uppsala-Sweden 2011: BBa_E1010
Spycatcher from addgene, Howarth lab: CnaB2114

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SpyCatcher	678,1067	feature/cds, CDS
mRFP	1,677	CDS, feature/cds
start	1,3	feature/start, start_codon

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1457000/1

Member of these Collections

• iGEM Parts Registry