Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shani Gal-Oz
Date created: 2014-10-14 11:00:00
Date modified: 2015-05-08 01:10:32
Human DsbA-L (GSTK1) gene
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1458005_sequence (Version 1) |
Description
DsbA-L is a protein present in the ER (endoplasmic reticulum). Its main action is a Glutathione S-Transferase action on the hormone adiponectin. It has the ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification, and it helps with binding two substrates with to s-s bonds and cellular detoxification.DsbA-L is expressed mostly in adipose tissue, and its expression level is negatively correlated with the obesity of mice and humans.
DsbA-L has been shown to bind adiponectin and increase the production of the HMW species, for that it can become a novel therapeutic and industrial target.
Notes
The DsbA-L sequence had iGEM Restriction sites that had to be change according to iGEM protocol.In position 131 G was changed to A to avoid Pst1 Site.
In position 446 A was changed to T to avoid Pst1 Site.
In position 467 A was changed to T to avoid Pst1 Site.
In position 545 C was changed to G to avoid Pst1 Site.
All changes in nucleotides was done according to an amino acid chart to maintain the same amino acids at the protein sequence Level.