BBa_K1459003

BBa_K1459003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-09-30 11:00:00

Date modified: 2015-05-08 01:10:32

PmrA-PmrB(LBT) two-component system

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1459003_sequence (Version 1)

Description

PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein.
In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.

Notes

We had to only add BioBrick Preffix and Suffix.

Source

PmrA-PmrB operon sequence was obtained thanks to kindness of prof. He from the University of Chicago.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1459003/1

Member of these Collections

• iGEM Parts Registry