BBa_K1459008

BBa_K1459008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:32

pmrC-GFP-terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1459008_sequence (Version 1)

Description

This is pmrC promoter from Salmonella enterica, with subsequent GFP and BBa_B1006 terminator. This part is one part of PmrA-PmrB detecting system. Upon phosphorylation by PmrB, PmrA binds to pmrC and induces expression of GFP.

Notes

We had to conduct directed mutagenesis to remove XbaI site from pmrC sequence.

Source

We obtained pmrC-GFP sequence thanks to kindness of prof. he from university of Chicago.

Components

Access	Instance	Definition
public	BBa_K1459006	BBa_K1459006
public	BBa_E0040	GFP
public	BBa_B1006	BBa_B1006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1459006	1,404	BBa_K1459006
BBa_E0040	411,1130	GFP
BBa_B1006	1139,1177	BBa_B1006

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1459008/1

Member of these Collections

• iGEM Parts Registry