Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grzegorz Ścibisz
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:32
pmrC-GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1459017_sequence (Version 1) |
Description
This is pmrC promoter from Salmonella enterica, with subsequent GFP. This part is one part of PmrA-PmrB detecting system. Upon phosphorylation by PmrB, PmrA binds to pmrC and induces expression of GFP. PmrB gains kinase activities after binding ions by it's extracellular loop (eg. iron or terbium atoms, depending on present binding tag).Notes
Apart from amplifying the sequence of interest we had to also perform a directed mutagenesis to eradicate XbaI site from pmrC sequence.Source
We obtained pmrC-GFP sequence thanks to kindness of prof. He from the University of Chicago.| Access | Instance | Definition |
| public public | BBa_K1459006 BBa_E0040 | BBa_K1459006 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1459006 BBa_E0040 | 1,46 53,772 | BBa_K1459006 GFP |