Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Holmes
Date created: 2014-08-13 11:00:00
Date modified: 2016-01-13 10:26:04
RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1460002_sequence (Version 1) |
Description
his part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with an appropriate promoter. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.Notes
The part contains a KpnI cut site between the biobrick prefix and the RBS that is a result of removing the T7 promoter from part BBa_K1460001.Source
This part is BBa_K1460001 with the T7 promoter removed.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61101 GST Linker Sequence CRS5 BBa_B1006 | 14,27 34,687 688,720 721,930 931,969 | feature/rbs ribosome_entry_site CDS feature/cds sequence_feature feature/misc feature/cds CDS feature/misc sequence_feature |