Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Holmes
Date created: 2014-08-13 11:00:00
Date modified: 2016-01-13 10:21:44
Anderson Promoter + NixA + Ter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1460003_sequence (Version 1) |
Description
This is a functional coding sequence for the protein NixA, which is a nickel transporter from H. pylori. This part will result in nickel intolerance from higher levels of intracellular nickel.Notes
The Anderson promoter provides strong constitutive expression of NixA in E. coli.Source
Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23102 BBa_J61101 NixA BBa_B1006 | 1,35 43,56 63,989 990,1028 | promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/cds feature/misc sequence_feature |