Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Holmes
Date created: 2014-08-25 11:00:00
Date modified: 2016-01-13 10:25:46
Anderson Promoter + MerT + MerP + ter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1460004_sequence (Version 1) |
Description
This is a functional coding sequence for the mercury transport proteins merT/merP from P. aeruginosa. This part will result in mercury intolerance due to greater mercury transport into engineered cells.Notes
The Anderson promoter provides strong constitutive expression of merT/merP in E. coliSource
Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa. This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61101 BBa_J23102 merT merP BBa_B1006 | 43,56 1,35 63,413 426,701 702,740 | feature/rbs ribosome_entry_site feature/promoter promoter feature/cds CDS CDS feature/cds feature/misc sequence_feature |