BBa_K1460005

BBa_K1460005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1460005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Holmes

Date created: 2014-08-25 11:00:00

Date modified: 2016-01-13 10:18:44

Anderson Promoter + RBS + CBP4

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1460005_sequence (Version 1)

Description

This is a functional coding sequence for the putative lead and nickel transport protein CBP4 from Nicotiana tabacum. It has been found that expression of this protein leads to increased nickel tolerance and lead intolerance.

Notes

The Anderson promoter will provide strong constitutive expression of the CBP4 gene in E. coli.

Source

Anderson promoter is that of part BBa_J23102. CBP4 is a naturally found coding sequence in Nicotiana tabacum.^[1] This part was synthesized by BioBasic and was transferred into pSB1C3 using standard cloning procedures.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23102	1,35	promoter, feature/promoter
BBa_J61101	43,56	ribosome_entry_site, feature/rbs
CBP4	63,2189	CDS, feature/cds

Other Properties

• igem#experience: Issues
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1460005/1

Member of these Collections

• //binding/metal
• iGEM Parts Registry