Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM14_Glasgow
Date created: 2014-10-14 11:00:00
Date modified: 2015-05-08 01:10:36
Recombinase Switch With GFP (weaker RBS)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1463002_sequence (Version 1) |
Description
PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter so that PhiC31 integrase switches on GFP expression. GFP contains a weaker ribosome binding site than in BBa_K1463001.Notes
The promoter had to face leftwards, the attP and attB sites had to be far enough apart to recombine efficiently and be in head-to-head (inverted repeat) orientation. GFP had to be to the right of the switch.Source
Composite biobrick consisting of attP, reversed BBa_J23100 promoter, forward facing BBa_B0010 terminator, PhiC31 attB site and the BBa_E0040 GFP gene with a medium strength BBa_B0032 ribosome binding site.| Access | Instance | Definition |
| public public public public public public public | BBa_K1463040 BBa_K1463030 BBa_K1463501 BBa_B0010 BBa_K1463041 BBa_B0032 BBa_E0040 | attP spacer rev promot BBa_B0010 attB BBa_B0032 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1463040 BBa_K1463030 BBa_K1463501 BBa_B0010 BBa_K1463041 BBa_B0032 BBa_E0040 | 1,54 63,73 82,116 125,204 213,267 276,288 295,1014 | attP spacer rev promot BBa_B0010 attB BBa_B0032 GFP |