BBa_K1473003

BBa_K1473003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1473003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan, Zhe
Date created: 2014-09-16 11:00:00
Date modified: 2015-05-08 01:10:39

gRNA 3



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K1473003_sequence (Version 1)

Description

This part is a 22 bp of gRNA(including PAM site) for CRISPR system. It targets Nalidixic acid resistance gene.

Notes

To make sure our design is valid for CRISPR system. We screened raw result (all 20bp sequences upstream to PAM sites) by calculating it's GC account and testing its specificity by bioinformatic tools.

Source

It is designed from nalR gene from bacteria JM101's genome sequence. The design is under basic rules of gRNA design: 20bp upstream from PAM site.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1473003/1