| Types | DnaRegion
|
| Roles | DNA
sequence_feature
|
| Sequences | BBa_K1473003_sequence (Version 1)
|
Description
This part is a 22 bp of gRNA（including PAM site） for CRISPR system. It targets Nalidixic acid resistance gene.
Notes
To make sure our design is valid for CRISPR system. We screened raw result (all 20bp sequences upstream to PAM sites) by calculating it's GC account and testing its specificity by bioinformatic tools.
Source
It is designed from nalR gene from bacteria JM101's genome sequence. The design is under basic rules of gRNA design: 20bp upstream from PAM site.
