BBa_K1473005

BBa_K1473005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1473005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shan, Zhe

Date created: 2014-09-16 11:00:00

Date modified: 2015-05-08 01:10:39

phagemid pBluescript

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K1473005_sequence (Version 1)

Description

This part is a phagemid. It functions as a normal plasmid when transformed into bacteria. But when helper phage M13KO7 infect this bacteria, it will produce progeny M13 phage.
Phagemid pBluescript contains a f1 ori while M13KO7 helper phage's package signal is modified as knock-down. So after the infection of M13KO7, its genome can produce protein but these proteins will see pBluescript as their true ???genome???. As a result, the progeny phage???s genome will only contains pBluescript, so they cannot produce next generation by infection.
We have removed some sequence on its MCS to create a linearized form of this phagemid.

Notes

Normally, a pBluescript contains EcoRI, PstI, XbaI and SpeI on its MCS. We have removed these sequence to create a linearized form of this phagemid.

Source

Its f1 ori comes from phage M13.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ori	460,1048	sequence_feature, feature/dna
f1 ori	2211,2666	sequence_feature, feature/dna
AmpR	1219,2079	CDS, feature/protein
AmpR promoter	2080,2185	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1473005/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors