BBa_K1473014

BBa_K1473014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1473014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan, Zhe
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:39

gRNA for NeoR/kanR 3



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K1473014_sequence (Version 1)

Description

This part is a 22 bp of gRNA for CRISPR system. It targets a gene can produce resistance to both neomycin and kanamycin.

Notes

This gRNA is designed based on basic rules of gRNA for CRISPR: 20bp upstream to PAM site

Source

From NeoR/kanR gene sequence of bacterial plasmid pSF-CMV-p15A Ori.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1473014/1