Types | DnaRegion
|
Roles | sequence_feature
DNA
|
Sequences | BBa_K1473016_sequence (Version 1)
|
Description
This part is a 22 bp of gRNA for CRISPR system. It targets a gene can produce resistance to both neomycin and kanamycin.
Notes
This gRNA is designed based on basic rules of gRNA for CRISPR: 20bp upstream to PAM site
Source
From NeoR/kanR gene sequence of bacterial plasmid pSF-CMV-p15A Ori.