BBa_K1484100

BBa_K1484100 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1484100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Guy Lewy

Date created: 2014-10-03 11:00:00

Date modified: 2015-06-10 02:14:31

4-hydroxybenzalacetone generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K1484100_sequence (Version 1)

Description

This part can be transformed into E. coli. When the transformants are incubated with p-coumaric acid and 50mM arabinose, they will metabolise the p-coumaric acid and secrete 4-hydroxybenzalacetone into the growth medium.

4-hydroxybenzalacetone is often referred to simply as benzalacetone.

Notes

Detectable amounts of 4-hydroxybenzalacetone were produced when 3mM p-coumaric acid was added to the transformants' growth medium. 9mM p-coumaric acid was also assayed and produced a larger amount of 4-hydroxybenzalacetone.

Beware the registry part labelled 'benzalacetone reductase'

Source

Originally from the genome of chinese rhubarb, rheum palmatum, cloned by the lab of Ikuro Abe at The School of Pharmaceutical Sciences, University of Shizuoka.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
AraC	1,1042	feature/operator, operator

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1484100/1

Member of these Collections

• //chassis/prokaryote/ecoli
• iGEM Parts Registry