Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angelina Munabi
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:41
P_SuI2, marchantia promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1484347_sequence (Version 1) |
Description
This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription under conditions of sulphur limitation. We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this.We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.
Notes
The removal of illegal restriction sites was considered but not completed due to direct interaction of the DNA with regulatory proteins. Also, it was ensured that the region selected for this part was directly upstream of the first ATG of the gene used to identify the promoter sequence. This was so that the 5' UTR, that is vital in plants, was maintained.Source
This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription under conditions of sulphur limitation. We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this.We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.