BBa_K1486009

BBa_K1486009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1486009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGem EPFL 2014
Date created: 2014-08-26 11:00:00
Date modified: 2015-05-08 01:10:42

CxpR & Split IFP1.4 [Nterm + Nterm]



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1486009_sequence (Version 1)

Description

This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2].

This construct was made in all orientations:

- CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008)

- IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010)

- CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)

Notes

All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.

Source

From Michnick Article. To Edit

igem#experience
Fails
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1486009/1