Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGem EPFL 2014
Date created: 2014-08-31 11:00:00
Date modified: 2015-05-08 01:10:42
CpxR-IFP[2] under pBAD
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1486013_sequence (Version 1) |
Description
This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).Notes
Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.Source
to edit| Access | Instance | Definition |
| public public public public public | BBa_I0500 BBa_B0034 BBa_K1486000 BBa_K1486004 BBa_K1486007 | pBad/araC BBa_B0034 BBa_K1486000 BBa_K1486004 BBa_K1486007 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_B0034 BBa_K1486000 BBa_K1486004 BBa_K1486007 | 1,1210 1211,1222 1223,1924 1925,1954 1955,2533 | pBad/araC BBa_B0034 BBa_K1486000 BBa_K1486004 BBa_K1486007 |