Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGem EPFL 2014
Date created: 2014-08-31 11:00:00
Date modified: 2015-05-08 01:10:42
IFP[1]-CpxR under pBAD
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1486014_sequence (Version 1) |
Description
This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).Notes
Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.Source
to edit| Access | Instance | Definition |
| public public public public public | BBa_I0500 BBa_B0034 BBa_K1486006 BBa_K1486004 BBa_K1486000 | pBad/araC BBa_B0034 BBa_K1486006 BBa_K1486004 BBa_K1486000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_B0034 BBa_K1486006 BBa_K1486004 BBa_K1486000 | 1,1210 1211,1222 1223,1627 1628,1657 1658,2359 | pBad/araC BBa_B0034 BBa_K1486006 BBa_K1486004 BBa_K1486000 |