Types | DnaRegion
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Roles | mature_transcript_region
RNA
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Sequences | BBa_K1487002_sequence (Version 1)
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Description
This part contains guide RNA (gRNA) that can target accessory colonization factor C (acfC) gene.
Notes
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
Source
Part was assembled using complementary primers to match a 20 base pair sequence in the acfC coding region.