Types | DnaRegion
|
Roles | RNA
mature_transcript_region
|
Sequences | BBa_K1487015_sequence (Version 1)
|
Description
This part contains guide RNA (gRNA) that can target toxin coregulated pilus P (tcpP) gene.
Notes
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
Source
Part was assembled using complementary primers to match a 20 base pair sequence in the tcpP coding region.