BBa_K1487025

BBa_K1487025 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1487025
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:43

Promoter + Guide RNA (gRNA) target for ctxA



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1487025_sequence (Version 1)

    Description

    This part contains guide RNA (gRNA) that can target cholera toxin A (ctxA) gene under strong constitutive promoter J23119.

    Notes

    Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

    Source

    Part was assembled using complementary primers to match a 20 base pair sequence in the ctxA coding region.

    Access Instance Definition
    public
    public
    		BBa_J23119
    BBa_K1487004
    		BBa_J23119
    BBa_K1487004

    Sequence Annotation Location Component / Role(s)
    BBa_J23119
    BBa_K1487004
    		1,35
    44,145
    		BBa_J23119
    BBa_K1487004

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1487025/1
     

    Attachments

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