BBa_K1487030

BBa_K1487030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1487030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Samantha Bermudez

Date created: 2014-10-02 11:00:00

Date modified: 2015-05-08 01:10:43

Promoter + Guide RNA (gRNA) target for tcpD

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1487030_sequence (Version 1)

Description

This part contains guide RNA (gRNA) that can target toxin coregulated pilus D (tcpD) gene under strong constitutive promoter J23119.

Notes

Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

Source

Part was assembled using complementary primers to match a 20 base pair sequence in the tcpD coding region.

Components

Access	Instance	Definition
public	BBa_J23119	BBa_J23119
public	BBa_K1487009	BBa_K1487009

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23119	1,35	BBa_J23119
BBa_K1487009	44,145	BBa_K1487009

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1487030/1

Member of these Collections

• iGEM Parts Registry