BBa_K1487041

BBa_K1487041 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1487041
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:43

Promoter + Guide RNA (gRNA) target for toxT



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1487041_sequence (Version 1)

    Description

    This part contains guide RNA (gRNA) that can target transcriptional regulator toxT (toxT) gene under strong constitutive promoter J23119.

    Notes

    Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

    Source

    Part was assembled using complementary primers to match a 20 base pair sequence in the toxT coding region.

    Access Instance Definition
    public
    public
    		BBa_J23119
    BBa_K1487020
    		BBa_J23119
    BBa_K1487020

    Sequence Annotation Location Component / Role(s)
    BBa_J23119
    BBa_K1487020
    		1,35
    44,145
    		BBa_J23119
    BBa_K1487020

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1487041/1
     

    Attachments

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