BBa_K1487042

BBa_K1487042 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1487042
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:43

Target sequence for acfA upstream from eYFP



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_K1487042_sequence (Version 1)

    Description

    This part contains a complementary target sequence for acfA that can be targeted by the gRNA from Part:BBa_K1487021. The target sequence is upstream from a coding region for enhanced yellow fluorescent protein (part: BBa_E0030).

    Notes

    Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

    Source

    Part was assembled using complementary primers to match a 20 base pair sequence in the acfA coding region.

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1487042/1
     

    Attachments

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