| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K1487052_sequence (Version 1)
|
Description
This part contains a 20bp complementary target sequence for toxin coregulated pilus E (tcpE) that can be targeted by the gRNA from Part:BBa_K1487031. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030).
Notes
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.
Source
Part was assembled using complementary primers to match a 20 base pair sequence in the tcpE coding region.
