BBa_K1487064

BBa_K1487064 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1487064
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:43

eYFP Reporter for acfB gRNA target with strong promoter.



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1487064_sequence (Version 1)

Description

This part contains a 20bp complementary target sequence for accessory colonization factor B (acfB) that can be targeted by the gRNA from Part:BBa_K1487022. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030). This reporter was placed under a strong constitutive reporter (Part:BBa_J23119).

Notes

Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.

Source

Part was assembled using complementary primers to match a 20 base pair sequence in the acfB coding region.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1487064/1