Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Bermudez
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:43
eYFP Reporter for acfD gRNA target with strong promoter.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1487066_sequence (Version 1) |
Description
This part contains a 20bp complementary target sequence for accessory colonization factor D (acfD) that can be targeted by the gRNA from Part:BBa_K1487024. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030). This reporter was placed under a strong constitutive reporter (Part:BBa_J23119).Notes
Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.Source
Part was assembled using complementary primers to match a 20 base pair sequence in the acfD coding region.| Access | Instance | Definition |
| public public | BBa_J23119 BBa_K1487045 | BBa_J23119 BBa_K1487045 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_K1487045 | 1,35 44,807 | BBa_J23119 BBa_K1487045 |