Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:45
Naringenin sensor (FdeR) with GFP as reporter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1497020_sequence (Version 1) |
Description
We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae.You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:
GFP fluorescence K1497020
mKate (DsRed variant) fluorescence K1497021
CFP fluorescence K1497022
You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
Notes
dSource
d| Access | Instance | Definition |
| public public | BBa_K1497019 BBa_E0040 | BBa_K1497019 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1497019 BBa_E0040 | 1,1214 1221,1940 | BBa_K1497019 GFP |