BBa_K1497197

BBa_K1497197 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1497197
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sascha Hein
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:46

B0034-CHI - Chalcone Isomerase from Petunia with strong RBS



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1497197_sequence (Version 1)

Description

The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.
For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.

Notes

no

Source

Petunia Hybrida

Access Instance Definition
public
BBa_B0034
BBa_B0034
Sequence Annotation Location Component / Role(s)
BBa_B0034
1,12
BBa_B0034
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1497197/1