Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Yang
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46
IPTG promoter-GFP (IG) backbone
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1500002_sequence (Version 1) |
Description
Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream. This part was primarily used as the initial plasmid into which mutator genes were cloned and site-directed mutagenesis was conducted. Also, it was intended to be the plasmid in which mutator genes were assembled in combinations before cloning the entire combination out into the AroF-mCherry-LVA backbone.Notes
The back two cut sites may be cut and mutator genes PCR'd from wild-type may then be inserted into this plasmid.Source
IPTG promoter was cloned from registry plasmid BBa_J04500, RBS.3 was designed into the primer for cloning IPTG, and these were cloned into reporter GFP, cutting the front two cut sites.| Access | Instance | Definition |
| public public | BBa_J04500 BBa_E0040 | BBa_J04500 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_E0040 | 1,220 227,946 | BBa_J04500 GFP |