BBa_K150007

BBa_K150007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K150007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pascal Kraemer
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:10:46

ColicinE1 operon



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K150007_sequence (Version 1)

Description

This colicinE1 operon contains all three important genes for colicinE1 expression and release. The colE1 gene codes for the lethal colicinE1 protein. The gene for the colE1 immunity protein located on the reverse strand is responsible for the immunity of the producing host bacteria to the colicinE1 and is therefore controlled by a constitutive promoter. Expression of the kil protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE1.

Notes

-

Source

This colicinE1 cassette is derived from the naturally existing colicinE1 plasmid out of the E. coli strain JC411.

Sequence Annotation Location Component / Role(s)
colE1 protein
colE1 immunity protein
kil protein
1,1569
1566,1907
1955,2092
feature/cds CDS
feature/cds CDS
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K150007/1