Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Renyao Wei
Date created: 2014-10-09 11:00:00
Date modified: 2016-01-13 12:56:38
RBS+smtA+mCherry
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1505001_sequence (Version 1) |
Description
The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. SmtA is a metallithionein isolated from E.coli and it binds covalently to divalent metal cation. It can usually binds 3-4 ions per protein, depending on the ion types. It is often used to increase the accumulation of metal ions intracellularly and potentially improve bacteria's tolerance of metal ions. The SmtA protein and mCherry protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of SmtA and for better visualization of successful transformation. The original use is to express SmtA in Magnetospirillum magneticum strain AMB-1.Notes
We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCherry protein is expected to improve the folding of SmtA (from E.coli) in AMB-1.Source
RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. smtA sequence is from BioBrick part BBa_K190021 (we modified the illegal PstI site).| Sequence Annotation | Location | Component / Role(s) |
| SmtA rbs (from pmsp3) mCherry ggghs linker | 66,233 20,47 249,959 234,248 | CDS feature/protein ribosome_entry_site feature/rbs CDS feature/protein feature/misc sequence_feature |