| Types | DnaRegion
|
| Roles | Coding
CDS
|
Description
Phosphotransferase system (PTS) is a major carbohydrate active -transport system, which is executed by a cascade of protein rally. For glucose uptake, IIA(Glc) is the only enzyme specific to glucose transport. We measure the concentration of glucose in the medium using catabolite repression mechanism toward lacI promoter activity upstream of a reporter gene, mRFP. We expect by over-expressing IIA(Glc) in E. coli, we will obtain different range of glucose standard curve in our measurement system.
Notes
The gene coding for IIA(Glc) from E.coli - crr - was isolated by colony PCR using primers with restriction sites of SpeI and PstI for forward primer and reverse primer, respectively.
Source
Escherichia coli DH5alpha genome
