| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1510010_sequence (Version 1)
|
Description
This part contains a strong promoter, J23100, and signal sequence yebF together with is recombinant red florescent protein, and a double terminator at the end. In our experiment, we use the circuit to compare and test if the signal sequence works.
Notes
In order to make red florescent protein(RFP), being secreted as recombinant protein with yebF, we design primer with special prefix and suffix(RFC23) between yebF and RFP in order to make this recombinant protein. At the end, we put it into pSB1C3, so it is standard biobrick compatible with EcoR1, Xba1, Spe1, and Pst1 cutting sites.
Source
The strong constitutive promoter, J23100 is from 2014 igem tool kit. At the down stream of promoter, yebF is a signal sequence, together with its own ribosomal binding site, from E.coli K12. After yebF, the red florescent sequence from igem 2014 tool kit is used. Finally, a commonly used igem from 2014 distribution double terminator, B0015, is constructed.
