| Types | DnaRegion
|
| Roles | oriT
Conjugation
|
| Sequences | BBa_K1510816_sequence (Version 1)
|
Description
A fusion of truncated INPNC anchor protein and C16 Streptococcus Mutants signal peptide becomes a bridge between E.coli and Streptococcus Mutants. This double-unit complex may not only provide a higher targeting specificity but also allow us to improve several cell-cell interactions, such as biofilm degrading and cell-holing. And in our cases, the cell-cell binding between our E.coli and Streptococcus Mutants may help us co-culture into the pathogenic-biofilm. The biofilm has long been accused of the most troublesome concrete barrier to surpass in organisms. The physical feature of biofilm often nests several pathogens, and its polysaccharide structure may serve as a safe shelter, preventing us from ridding problems from the root. However, we are flipping the game this time. With the aid of K1510816, our E.coli can attach on the Streptococcus Mutants, and be co-cultured into biofilm, giving us the chance to challenge the unsolved oral-biofilm problem.
Notes
Make sure the tail linker of INPNC sequence can match the C16 prefix.
Source
We truncate K523013 of 2011 Edinberg team and derive C16 from the genome of Streptococuus Mutants UA159.
