BBa_K1510820

BBa_K1510820 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1510820

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kao Jung Chang

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:48

RFP indicated INPNC performance evaluation

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_K1510820_sequence (Version 1)

Description

We anneal a RFP at the end of our INPNC display gene, in the hope that under the measure of fluorescence light and O.D value, we can derive pure INPNC expression quantity.

Notes

We consider the E1010 performance in the aspect of its efficiency and its DNA size, in afraid of giving gene express too much burden.

Source

We truncated INPNC ourselves, derive C16 from Streptococcus Mutants and we ligate an igem BBa_E1010 RFP at the tail of this surface display gene. To round this gene up, we gave it a terminator B0015.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	611,613	feature/rbs, ribosome_entry_site
plac+lacz	38,581	promoter, feature/promoter
INPNC	632,1555	CDS, feature/protein
C16	1609,1659	CDS, feature/protein
RFP	1666,2371	feature/protein, CDS
terminator	2372,2500	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1510820/1

Member of these Collections

• iGEM Parts Registry