Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kao Jung Chang
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:48
Fragrance generator part1: ketoisocaproate decarboxylase (THI3) and RBS
| Types | DnaRegion |
| Roles | oriT Conjugation |
| Sequences | BBa_K1510922_sequence (Version 1) |
Description
encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction.BBa_B0034(RBS)can not only facilitate the transcription rate but also bind to the other two fragrance generators.Notes
To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.Source
THI3 gene from Saccharomyces cerevisiae and BBa_B0034 from 2014 igem distribution.| Access | Instance | Definition |
| public public | BBa_B0034 BBa_K1510087 | BBa_B0034 BBa_K1510087 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_K1510087 | 1,12 19,1848 | BBa_B0034 BBa_K1510087 |