BBa_K1510922

BBa_K1510922 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1510922

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kao Jung Chang

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:48

Fragrance generator part1: ketoisocaproate decarboxylase (THI3) and RBS

Details

• Types: DnaRegion
• Roles: oriT; Conjugation
• Sequences: BBa_K1510922_sequence (Version 1)

Description

encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction.BBa_B0034(RBS)can not only facilitate the transcription rate but also bind to the other two fragrance generators.

Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.

Source

THI3 gene from Saccharomyces cerevisiae and BBa_B0034 from 2014 igem distribution.

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_K1510087	BBa_K1510087

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_K1510087	19,1848	BBa_K1510087

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1510922/1

Member of these Collections

• iGEM Parts Registry